Science & Discovery: Discover the Globe Through Research and Advancement
- LD-2P-FLIM provides a large field of view, dual region platform for multiparameter quantitative brain function imaging.
- Integrates two-photon fluorescence lifetime imaging for depth resolved, concentration independent biochemical and functional contrasts.
- Dual region acquisition enables simultaneous monitoring of spatially separated cortical areas for correlated neural and vascular dynamics.
- Instrumentation advances include a field programmable entrance array module and industrial two-photon objectives to expand imaging throughput and quality.
- In vivo validation used Thy1-GFP-M and CX3CR1-GFP mice; study supported by national grants and declares no competing interests.
Entry background
Gotten : December 17, 2025
Accepted : July 1, 2026
Published on-line : August 7, 2026
Released in concern : August 11, 2026
Acknowledgments
We give thanks to Prof. Yang Zhan (Mind Cognition and Mind Disease Institute, Shenzhen Institutes of Advanced Modern Technology, Chinese Academy of Sciences, Shenzhen, China) and Prof. Bo Peng (Institute for Translational Brain Research Study, Fudan University) for giving the Thy 1 -GFP-M and CX 3 CR 1 -GFP mice, specifically. We thank Kui Wu and Yujie Zhu (Shenzhen Boray Modern Technology, Ltd.) for giving the ideas of developing the field programmable entrance arraymodule. We give thanks to Hui Zhang (Nanjing Donglilai Optics & & Electronic Devices Business Co., Ltd.) for gauging the imaging capacities of industrial two-photon purposes. This work was sustained by the National Key R & D Program of China (2023 YFF 0715200, the Shenzhen Medical Research Fund (D 2404004, National Natural Science Foundation of China (81927803, 62105353, 92359303, 62305369, 62405351, and 62475278, the Life Sciences Foundation of Guangdong District (2020 B 121201010 and 2024 A 1515012517, the Shenzhen Basic Study Program (QNXMB 20250701095915022, KJZD 20240903095718024, JCYJ 20241202124924033, RCJC 20200714114433058, JCYJ 20230807140819041, ZDSY 20130401165820357, and JCYJ 20220531100802004 and Youth Innovation Promotion Organization of the Chinese Academy of Sciences (2023377
Author contributions
S.Y., J. Yu, J. Yao, H.L., Y. Guo, J.C., H.Z., C.L., and W.Z. made study; S.Y., Y. Gao, M.D., Y.S., X.X., N.X., H.X., T.W., and Y.H. performed study; L.L., J.Z., M.X., and L.Z. added new reagents/analytic devices; S.Y., Y. Gao, M.D., and X.C. assessed information; and S.Y., M.D., and W.Z. wrote the paper.
Competing rate of interests
The writers proclaim no completing interest.
Notes
This write-up is a PNAS Direct Entry.
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