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- Spatial-EV-seq enables spatially resolved mapping of extracellular vesicles within intact tissue sections.
- Integrates spatial transcriptomics with targeted molecular labeling and rolling circle amplification for high-sensitivity EV RNA and protein detection.
- Spatially resolved data uncovers tissue-location dependent heterogeneity and distinct EV subpopulations, including immunomodulatory PD-L1-positive vesicles.
- Facilitates translational applications in diagnosis, predicting immunotherapy response, and guiding targeted EV-based therapeutic strategies.
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